Part:BBa_K268020

YGR213C -> GFP

~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YGR213C gene. For transformation in S. cerevisiae only, this part will swap out the YGR213 gene and replace it with a Kan marker+GFP coding region downstream of the YGR213C promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.

This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers: YGR213Cdel-F: 62-mer GTATGTAATAGACAGTCAGCACTTTTCACCACTTCGAACATGcggatccccgggttaattaa YGR213Cdel-R: 61-mer GAATAAAACCACATTTCCCACATGTAAATATAGAAAGCAGTgaattcgagctcgtttaaac

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2461
  Illegal BglII site found at 1015
  Illegal BamHI site found at 44
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 704